Antibiotic Resistance Platform
The Antibiotic Resistance Platform (ARP) is a cell-based array of mechanistically distinct individual resistance elements in an identical genetic background. The ARP consists of resistance elements to 18 classes of antibiotics, represented by the inner wheel. The middle wheel divides the 18 classes into individual antibiotics found within each class. The outer wheel depicts the array of resistance genes within the ARP, grouped based on antibiotic substrate specificity. There are over 100 antibiotic resistance genes currently in the ARP.
The Minimal Antibiotic Resistance Platform
The Minimal Antibiotic Resistance Platform (ARP) is a tool that can be used for the general dereplication of antibiotics in natural product extracts. This platform consists of fifteen low-copy number plasmids that constitutively express individual resistance genes that target seventeen of the most commonly found antibiotics. This kit is useful for antibiotic dereplication and adjuvant discovery. A map of the genes included in the minimal ARP is below.
The Minimal ARP is available to order from Addgene here.
To learn more about antibiotic dereplication, check out our publication in the Journal of Visualized Experiments here.
Selected Publications
