Alison enjoys playing soccer, reading and spending time in the kitchen baking and trying new recipes.
Aminoglycosides are used to treat both Gram-negative and Gram-positive bacterial infections by targeting the ribosome and preventing protein translation. One mechanism of resistance to most forms of these antibiotics occurs through modification of the ribosome by the methyltransferase ArmA. My project involves high-throughput cell-based screening of natural products to discover novel inhibitors of ArmA and restore aminoglycoside activity against resistant organisms.
The Comprehensive Antibiotic Resistance Database is a collection of known resistance genes and tools to analyze and predict antibiotic resistomes from protein or nucleotide sequence. These genes have been assembled as probes on an array to capture resistance profiles in a variety of clinical and environmental samples. My project aims to evaluate and validate the detection and specificity of this array using various strains with known resistance profiles.